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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
27/01/2020 |
Actualizado : |
29/05/2020 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
BERNASCHINA, Y.; LEONI, C.; ALANIZ, S. |
Afiliación : |
YESICA STEFANIA BERNASCHINA CORREA, Departamento de Protección Vegetal, Fitopatología, Facultad de Agronomía, Universidad de la República, Uruguay; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINA LEONI VELAZCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SANDRA ALANIZ, Departamento de Protección Vegetal, Fitopatología, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay. |
Título : |
Genetic diversity evidence a mixed reproduction mode in Venturia oleaginea populations in Uruguay. |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
Journal of Plant Pathology, 1 February 2020, Volume 102, Issue 1, Pages 123-133. Doi: https://doi.org/10.1007/s42161-019-00396-2 |
ISSN : |
1125-4653 |
DOI : |
10.1007/s42161-019-00396-2 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 15 June 2018 / Accepted: 3 September 2019 / Published online: 17 October 2019.
Funding text: This research was funded by The National Institute of Agricultural Research (INIA - Uruguay). The first author granted a scholarship from The National Agency for Research and Innovation, Uruguay (ANII grant POS_NAC_2014_1_102182) and from UdelaR to carry out this research as part of her Master Degree. The authors are grateful to Dr. Guillermo Perez for his substantial contributions along the research process, particularly in analysis and discussion of genetic diversity data. Also we are grateful to Paula Conde and Jose Villamil for their support in contacting the surveyed orchards and to the owners of olive orchards, and to Pedro Mondino for his assistance in obtaining monoconidial isolates. Partial results were presented at XXV Congress Sociedad Chilena de Fitopatología ? XIX Congreso Asociación Latinoamericana de Fitopatología y LVII APS Caribbean Division Meeting, Termas de Chillán, Chile, 2-5 October, 2017. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
Contenido : |
ABSTRACT.
Olive scab caused by Venturia oleaginea is one of the most important diseases of olive worldwide. The fungus infects young leaves and fruits by asexually produced conidia. The sexual stage and its importance in the epidemiology and genetics of the pathogen population is unknown. A collection of 52 fungal isolates was obtained from scabbed leaves collected in 16 orchards from three major regions in Uruguay. All isolates were identified as V. oleaginea by PCR with specific primers and sequence analysis of the TEF-1α gene region. Five colony morphotypes and different growing rates were observed on potato dextrose agar (PDA) medium. Universally primed polymerase chain reaction (UP-PCR) was successfully used for assessing the genetic diversity and population structure of the V. oleaginea isolates, whereas ISSR was unable to generate reproducible and polymorphic bands. Two populations genetically different were identified: [1] composed by 21 isolates mainly from Southern and South-Western regions; [2] composed by 31 isolates mainly from South-Eastern and Southern regions. Both populations showed a moderate gene diversity expressed as hNei = 0.163 and 0.212 for population [1] and [2], respectively. There was evidence of linkage disequilibrium in both populations, rejecting the null hypothesis of random mating as the predominant reproduction mode, a typical result of clonal populations. However, all isolates were genotypically different, an indirect evidence of recombination. We conclude that V. oleaginea populations in Uruguay present a mixed mode of reproduction, a characteristic of many pathogens that are difficult to control.
© 2019, Società Italiana di Patologia Vegetale (S.I.Pa.V.). MenosABSTRACT.
Olive scab caused by Venturia oleaginea is one of the most important diseases of olive worldwide. The fungus infects young leaves and fruits by asexually produced conidia. The sexual stage and its importance in the epidemiology and genetics of the pathogen population is unknown. A collection of 52 fungal isolates was obtained from scabbed leaves collected in 16 orchards from three major regions in Uruguay. All isolates were identified as V. oleaginea by PCR with specific primers and sequence analysis of the TEF-1α gene region. Five colony morphotypes and different growing rates were observed on potato dextrose agar (PDA) medium. Universally primed polymerase chain reaction (UP-PCR) was successfully used for assessing the genetic diversity and population structure of the V. oleaginea isolates, whereas ISSR was unable to generate reproducible and polymorphic bands. Two populations genetically different were identified: [1] composed by 21 isolates mainly from Southern and South-Western regions; [2] composed by 31 isolates mainly from South-Eastern and Southern regions. Both populations showed a moderate gene diversity expressed as hNei = 0.163 and 0.212 for population [1] and [2], respectively. There was evidence of linkage disequilibrium in both populations, rejecting the null hypothesis of random mating as the predominant reproduction mode, a typical result of clonal populations. However, all isolates were genotypically different, an indirect evidence of recomb... Presentar Todo |
Palabras claves : |
Fusicladium oleagineum; Genetic diversity; Olive scab. |
Thesagro : |
OLEA EUROPAEA; OLIVOS. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
Marc : |
LEADER 03666naa a2200241 a 4500 001 1060692 005 2020-05-29 008 2020 bl uuuu u00u1 u #d 022 $a1125-4653 024 7 $a10.1007/s42161-019-00396-2$2DOI 100 1 $aBERNASCHINA, Y. 245 $aGenetic diversity evidence a mixed reproduction mode in Venturia oleaginea populations in Uruguay.$h[electronic resource] 260 $c2020 500 $aArticle history: Received: 15 June 2018 / Accepted: 3 September 2019 / Published online: 17 October 2019. Funding text: This research was funded by The National Institute of Agricultural Research (INIA - Uruguay). The first author granted a scholarship from The National Agency for Research and Innovation, Uruguay (ANII grant POS_NAC_2014_1_102182) and from UdelaR to carry out this research as part of her Master Degree. The authors are grateful to Dr. Guillermo Perez for his substantial contributions along the research process, particularly in analysis and discussion of genetic diversity data. Also we are grateful to Paula Conde and Jose Villamil for their support in contacting the surveyed orchards and to the owners of olive orchards, and to Pedro Mondino for his assistance in obtaining monoconidial isolates. Partial results were presented at XXV Congress Sociedad Chilena de Fitopatología ? XIX Congreso Asociación Latinoamericana de Fitopatología y LVII APS Caribbean Division Meeting, Termas de Chillán, Chile, 2-5 October, 2017. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. 520 $aABSTRACT. Olive scab caused by Venturia oleaginea is one of the most important diseases of olive worldwide. The fungus infects young leaves and fruits by asexually produced conidia. The sexual stage and its importance in the epidemiology and genetics of the pathogen population is unknown. A collection of 52 fungal isolates was obtained from scabbed leaves collected in 16 orchards from three major regions in Uruguay. All isolates were identified as V. oleaginea by PCR with specific primers and sequence analysis of the TEF-1α gene region. Five colony morphotypes and different growing rates were observed on potato dextrose agar (PDA) medium. Universally primed polymerase chain reaction (UP-PCR) was successfully used for assessing the genetic diversity and population structure of the V. oleaginea isolates, whereas ISSR was unable to generate reproducible and polymorphic bands. Two populations genetically different were identified: [1] composed by 21 isolates mainly from Southern and South-Western regions; [2] composed by 31 isolates mainly from South-Eastern and Southern regions. Both populations showed a moderate gene diversity expressed as hNei = 0.163 and 0.212 for population [1] and [2], respectively. There was evidence of linkage disequilibrium in both populations, rejecting the null hypothesis of random mating as the predominant reproduction mode, a typical result of clonal populations. However, all isolates were genotypically different, an indirect evidence of recombination. We conclude that V. oleaginea populations in Uruguay present a mixed mode of reproduction, a characteristic of many pathogens that are difficult to control. © 2019, Società Italiana di Patologia Vegetale (S.I.Pa.V.). 650 $aOLEA EUROPAEA 650 $aOLIVOS 653 $aFusicladium oleagineum 653 $aGenetic diversity 653 $aOlive scab 700 1 $aLEONI, C. 700 1 $aALANIZ, S. 773 $tJournal of Plant Pathology, 1 February 2020, Volume 102, Issue 1, Pages 123-133. Doi: https://doi.org/10.1007/s42161-019-00396-2
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
20/01/2022 |
Actualizado : |
20/01/2022 |
Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
Autor : |
DEL CAMPO, M.; TOYOS, G.; ALBÍN, F.; BORCA, A.; CORREA, D.; ROBAINA, R.; BRITO, G.; SOARES DE LIMA, J.M. |
Afiliación : |
MARCIA DEL CAMPO GIGENA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; G. TOYOS, INAC (Instituto Nacional de Carnes), Rincón 545, Montevideo (Uruguay); F. ALBÍN, INAC (Instituto Nacional de Carnes), Rincón 545, Montevideo (Uruguay); A. BORCA, INAC (Instituto Nacional de Carnes), Rincón 545, Montevideo (Uruguay); DANIELA CORREA NACIMENTO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RICARDO ROBAINA, INAC (Instituto Nacional de Carnes), Rincón 545, Montevideo (Uruguay); GUSTAVO WALTER BRITO DIAZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN MANUEL SOARES DE LIMA LAPETINA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Third uruguayan national beef quality audit: bruises characterization. |
Fecha de publicación : |
2021 |
Fuente / Imprenta : |
In: AUPA, Proceedings del VII Congreso Uruguayo de Producción Animal. Sección Una Sola Salud (Single Health Section), 14 y 15 diciembre 2021. Archivos Latinoamericanos de Producción Animal, 29(Supl.1), p.119-121. |
Serie : |
(Archivos Latinoamericanos de Producción Animal, Vol.29, Supl.1) |
ISSN : |
1022-1301; online 2075-8359 |
Idioma : |
Inglés |
Notas : |
Corresponding author: M. del Campo, Instituto Nacional de Investigación Agropecuaria (INIA), Tacuarembó, Ruta 5 km 386 (Uruguay), mailto:mdelcampo@inia.org.uy |
Contenido : |
The third Uruguayan National Beef Quality Audit (UNBQA) was held in a cooperative project among Colorado State University (CSU), INAC e INIA, assessing breed-type, brands, horns and mud/manure, bruises, offal condemnation and carcass quality. Bruises are avery good indicator of animal welfare and when a bruise affects muscle tissue, the affected area is trimmed, leading to economic losses. Many of the UNBQA-2002, 2007 and 2013 findings were used to implement training practices related with animal welfare and pre slaughter handling for all the stakeholders of the Uruguayan meat chain. |
Palabras claves : |
Animal welfare; Bruises; Preslaughter period stress. |
Asunto categoría : |
L01 Ganadería |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16217/1/2951-Article-Text-10069-4-10-20211213-10.pdf
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Marc : |
LEADER 01705nam a2200265 a 4500 001 1062674 005 2022-01-20 008 2021 bl uuuu u01u1 u #d 022 $a1022-1301; online 2075-8359 100 1 $aDEL CAMPO, M. 245 $aThird uruguayan national beef quality audit$bbruises characterization.$h[electronic resource] 260 $aIn: AUPA, Proceedings del VII Congreso Uruguayo de Producción Animal. Sección Una Sola Salud (Single Health Section), 14 y 15 diciembre 2021. Archivos Latinoamericanos de Producción Animal, 29(Supl.1), p.119-121.$c2021 490 $a(Archivos Latinoamericanos de Producción Animal, Vol.29, Supl.1) 500 $aCorresponding author: M. del Campo, Instituto Nacional de Investigación Agropecuaria (INIA), Tacuarembó, Ruta 5 km 386 (Uruguay), mailto:mdelcampo@inia.org.uy 520 $aThe third Uruguayan National Beef Quality Audit (UNBQA) was held in a cooperative project among Colorado State University (CSU), INAC e INIA, assessing breed-type, brands, horns and mud/manure, bruises, offal condemnation and carcass quality. Bruises are avery good indicator of animal welfare and when a bruise affects muscle tissue, the affected area is trimmed, leading to economic losses. Many of the UNBQA-2002, 2007 and 2013 findings were used to implement training practices related with animal welfare and pre slaughter handling for all the stakeholders of the Uruguayan meat chain. 653 $aAnimal welfare 653 $aBruises 653 $aPreslaughter period stress 700 1 $aTOYOS, G. 700 1 $aALBÍN, F. 700 1 $aBORCA, A. 700 1 $aCORREA, D. 700 1 $aROBAINA, R. 700 1 $aBRITO, G. 700 1 $aSOARES DE LIMA, J.M.
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